JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ916883.1
Database:	nt	Go to original record
Description:	Phyllanthera grayi voucher Forster 24232 (BRI) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phyllanthera grayi
Original size:	1682 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	792	1353	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctatttta cacaaacaaa ggttcagaaa
151   acgaaaacga ggataggtgc agagactcga cggaagatgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcctt ccaaaattca gaaaggatga
251   aggataaaca catatacata cgtattgaat actatatcaa atgattatgc
301   cgacccgaat gaatctgtat tttttctata aaatacggaa gaattggtgt
351   gagtcgattc tttcttcaat gtggaatcga atattcattg atcaaatgat
401   tcactccata gtctgtagat ccacttttca agaacggatt aatcggacga
451   gaataaagat agagtcccgt tctacatgtc aatgctggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttaa aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details