JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ916878.1
Database:	nt	Go to original record
Description:	Mondia whitei voucher ex hort. Ollerton s.n., trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Mondia whitei
Original size:	1666 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	793	1338	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctatttta cacaaacaaa ggataggtgc
151   agagactcga cggaagatgt tctaacaaat ggagttggcc gcgttggtag
201   agaaatcctt ccatcgaaaa ttcagaaagg atgaaggata aacacatata
251   catacgtatt gaatactgta tcaaatgatt atgccgaccc gaatgaatct
301   gtattttttc tataaaaaac ggaagaattg gtgtgagtcg attctttctt
351   caatgcggaa tcgaatattc attgatcaaa tgattcactc catagtctgt
401   agatcccctt ttcaagaacg gattaatcgg acgagaataa agatagagtc
451   ccgttctaca tgtcaatgct ggcaacaatg aaatttatag taagaggaaa
501   atccgtcgac ttaaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details