JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ916874.1
Database:	nt	Go to original record
Description:	Ectadium latifolium voucher Albers 2413 (UBT) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ectadium virgatum
Original size:	1680 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	792	1351	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctatttta cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagatgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcctt ccatcgaaaa ttcagaaagg
251   atgaagcaca tatacatacg tattgaatac tctatcaaat gattatgccg
301   acccgaatga atccgtattt tttctataaa aaacggaaga attagtgtga
351   gtcgattctt tcttcaatgt ggaatcgaat attcattgat caaatgattc
401   actccatagt ctgtagatcc ccttttcaag aacggattaa tcggacgaga
451   ataaagatag agtcctgttc tacatgtcaa tgctggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgacttaaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details