JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688851.1
Database:	nt	Go to original record
Description:	Cordia decandra voucher 1873 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cordia decandra
Original size:	1030 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	23	594	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttc caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa agaaaggttc
151   agaaagcgaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtcgaggaat ctttccatca aaacttcaga
251   aaggatgaag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gaaatgatta atgacgaccc taatctgctt tttttatgaa aaaaaaaagg
351   aaaagttggt gtgaatcgat tccacattga agaaagaatc gaagattcat
401   tgatcaaata attcactcca tagtctgata gatcttttga agaactaatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details