JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688842.1
Database:	nt	Go to original record
Description:	Heliotropium paronychioides voucher 2241 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Heliotropium paronychioides
Original size:	1000 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	578	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa agaaaggttc
151   agaaagcaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggggtt gactgcattg gtcgaggaat ctttctatcg agacttcaga
251   aaggatgaag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gaaatgatta ataacgaccc taatctgttc tgtttttttt ttatgaaaaa
351   aaaggaagaa ttggtgtgaa tcgattccac attgaagaaa gaaccgaata
401   ttcattgatc aaatcactca ctccatagtc tgatagttct tttgaagaac
451   tgattaatcg gacgagaata aagatagagt cccgttctac atgtcaatac
501   cggcaacaat gaaatttata gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details