JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688841.1
Database:	nt	Go to original record
Description:	Heliotropium krauseanum voucher 8779 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Heliotropium krauseanum
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	582	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa agaaaggttc
151   agaaagcaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggggtt gactgcattg gtcgaggaat ctttctatcg agacttcaga
251   aaggatgaag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gaaatgatta atgacgaccc caatctgttc tgtttttttt atgaaaaaaa
351   aggaagaatt ggtgtgaatc gattccacat tgaagttgaa gaaagaaccg
401   aatattcatt gatcaaatca ctcactccat agtctgatag ttcttttgaa
451   gaactgatta atcggacgag aataaagata gagtcccgtt ctacatgtca
501   ataccggcaa caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details