JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688807.1
Database:	nt	Go to original record
Description:	Tiquilia paronychioides voucher 8798 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tiquilia paronychioides
Original size:	943 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	567	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa aaaagcgaaa
151   aaaggatagg tgcagagact cgatggaagc tgttctaaca aatggagttg
201   actgccttgg tcgaggaatc tttccatcga aacttccgaa agggtggagg
251   ataaacgtat atacatatgt attcaatact ctatcaaatg aaatgattaa
301   tgatgaccct aatctgattt ttttatatta tgaaaaaaaa aagaaagagt
351   tggtctgagt tgattccaca ttgaagaaag ggtcgaatat tcattgatca
401   aatcattcac tccataatct gatagatctt ttgaagaact gattaatcgg
451   acgagaataa agatagagtc ccgttctaca tgtcaatacc ggcaaaaatg
501   aaatttatag taagaggaaa atccgtcgat ttaaaacatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details