JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688804.1
Database:	nt	Go to original record
Description:	Heliotropium nicotianifolium voucher 53890 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Heliotropium nicotianifolium
Original size:	1019 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	576	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aacgggcaat cctgagccaa atcctgtttt ccgaaaacaa agaaaggttc
151   agaaagcaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggggtt gactgcattg gtcgaggaat ctttctatcg agacttcaga
251   aaggatgaag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gaaatgatta atgacgaccc taatctgttc tgtttttttt akraaaaaaa
351   aggaagaatt ggtgtgaatc gattccacat taaagaaaga accgaatatt
401   cattgatcaa atcactcact ccatagtctg atagctcttt tgaagaactg
451   attaatcgga cgagaataaa gatagagtcc cgttctacat gtcaataccg
501   gcaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details