JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688803.1
Database:	nt	Go to original record
Description:	Euploca campestris voucher 53873 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Euploca campestris
Original size:	964 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	523	+	522 bp

Amplicon:

522 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa aaaaaggttc
151   agaaagcgaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggggt tgactgcatt tgcattggtc gaggaatctt tccaccgaaa
251   cttcagaaag gataatgacg cggctaattt ttttttttca taaaaaaaag
301   aagagttgat gtgaatcgat tccacattga agaaagaatt gaatattcat
351   tgatcaaacc attcactcca tagtctgata gatcttttga agaactgatt
401   aatcagacga gaataaagat agagtcccgt tctacatgcc aataccggca
451   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
501   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	522	503	2.35e-08

Match details