JRC GMO-Amplicons | EURL GMFF

Record ID:	EF688800.1
Database:	nt	Go to original record
Description:	Myriopus salzmannii voucher 53848 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Myriopus salzmannii
Original size:	988 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	568	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atccggtttt ctgaaaatta agaaaggttc
151   agaaagcgat aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggggtt gactgtattg gtcgaggaat ctttccaccg aaacttcaga
251   aaggatgaag gataaacata tatatatatg tattccatac tctatcaaat
301   gaaatgatta atgacgagcc taattttttt ttttatgaaa aaaggaagag
351   ttggtgtgaa tcgattccac attgaagaaa gaatcgaata ttcattgatc
401   aaaccattca ctccatagtc tgatagatct tttgaagaac tgattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatac cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details