JRC GMO-Amplicons | EURL GMFF

Record ID:	EF673700.1
Database:	nt	Go to original record
Description:	Photinia ardisiifolia voucher H. L. Cheng 016 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Photinia serratifolia var. ardisiifolia
Original size:	604 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5	594	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttatg aaaataaaca agggtttcat
151   aaaccgaaaa taaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgca ttgtgttagt aaaggaatcc ttccatcgaa
251   acttcagaaa ggatgaagga taaacctata tacatacgta tagtactgaa
301   atactatctc aaaatgatta atgacgaccc aaatctgtat ttttttatat
351   ttatatgaaa aatgaaagac ttgttgtgaa tcgattaaaa attgaaaaaa
401   gaatcgaata ttcattgatc aaaccattca ctccaccgta gtctgataga
451   tctttttact aattgattaa tcggatgaga ataaagatag agtcccgtta
501   tacatgttaa tatcgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details