JRC GMO-Amplicons | EURL GMFF

Record ID:	EF673698.1
Database:	nt	Go to original record
Description:	Photinia lasiopetala voucher H. L. Cheng 025 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Photinia serratifolia var. lasiopetala
Original size:	603 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5	593	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttatg aaaataaaca agggtttcat
151   aaaccgaaaa taaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgca ttgtgttagt aaaggaatcc ttccatcgaa
251   acttcagaaa ggatgaagga taaacctata tacatacgta tagtactgaa
301   atactatctc aaaatgatta atgacgaccc aaatctttat tttttatatt
351   tatatgaaaa atgaaagact tgttgtgaat ctattaaaaa ttgaaaaaag
401   aatcgaatat tcattgatca aaccattcac tccaccgtag tctgatagat
451   ctttttaata attgattaat cggacgagaa taaagataga gtcccattat
501   acatgttaat atcgacaaca atgaaattta tagtaagagg aaaatccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details