Match details

Record ID: EF673697.1
Database: nt Go to original record
Description: Photinia serratifolia var. daphniphylloides voucher H. L. Cheng 013 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon: Photinia serratifolia var. daphniphylloides
Original size: 610 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5 600 + 596 bp

Amplicon: 596 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccttgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttatg aaaataaaca agggtttcat
151   aaaccgaaaa taaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgca ttgtgttagt aaaggaatcc ttccatcgaa
251   acttcagaaa ggatgaagga taaacctata tacatacata tagtactgaa
301   atactatctc aaaatgatta atgacgaccc aaatctgtat tttttatatt
351   tatatgaaaa atgaaagact tgttgtgaat cgattaaaaa ttgaaaaaag
401   aatcgaatat tcattgatca aaccattcac tccaccgtag tctgatagat
451   ctttttaata attgattaat cggacgagaa taaagataga gtcccattat
501   acatgttaat atcgacaaca atgaaattta tagtaagagg aaaatccgtc
551   gactttcgaa atcggtagac gctacggttc aagtccctct atcccc 

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.68e-08
primerA 100.0 20 bp 0 0 557 576 2.68e-08
primerB 100.0 20 bp 0 0 596 577 2.68e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.