JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ787752.1
Database:	nt	Go to original record
Description:	Viola dissecta voucher KWNU56518 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viola dissecta
Original size:	954 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	549	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   agggcaatcc tgagccaaat ccgatttttt gaaatgaaaa aagacagaat
151   aaataaaaaa ggataggtgc agagactcaa tggaagctgt tctaataaat
201   ggagttgact gtcttgcatt agttaaagta agggaaccga acctttcgtt
251   aaaattgcgg attcaaagta aagtataacc ctataaatac atatacgtac
301   tgaaaaacta tctcaaataa ttaatgtaat tatgaaaaat aaaataaaaa
351   tattgaatcg atttcaagtt gaagaaaaaa tcgattgatc aaatcattca
401   ctccacagtc tgataaataa ctaattaatc ggacgagaat aaagatagag
451   tcccattcta catgttaata ttgacaacaa ggaaatttat agtaagagga
501   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details