JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ787751.1
Database:	nt	Go to original record
Description:	Viola eizanensis voucher KWNU56523 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viola eizanensis
Original size:	960 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	555	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggcaatcc tgagccaaat ccgatttttt gaaatgaaaa aaaaagttta
151   tatagacaga ataaataaaa aaggataggt gcagagactc aatggaagct
201   gttctaataa atggagttga ctgtcttgca ttagtaaagt aagggaacct
251   ttcgttaaaa ttgcggattc aaagtaaagg ataaccctat aaatacatat
301   acgtactgaa agactatctc aaataattaa tgtaattatg aaaaataaaa
351   taaaaatatt gaatcgattt caagttgaag aaaaaatcga ttgatcaaat
401   cattcactcc acagtctgat aaataactaa ttaatcggac gagaataaag
451   atagagtccc attctacatg ttaatattga caacaaggaa atttatagta
501   agaggaaaat ccgtcgactt tagaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details