JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ787747.1
Database:	nt	Go to original record
Description:	Viola chaerophylloides voucher KWNU57083 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viola chaerophylloides
Original size:	970 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	564	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggcaatcc tgagccaaat ccgatttttt gaaatgaaaa aaagtttata
151   tagtatatag acagaataaa taaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aataaatgga gttgactgtc ttgcattagt aaagtaaggg
251   aacctttcgt taaaattgcg gattcaaaca aagtaaagga taaccctaga
301   aatacatata cgtactgaaa gactatctca aataattaat gtaattatga
351   aaaataaaat aaaaatattg aatcgatttc aagttgaaga aaaaatcgat
401   tgatcaaatc attcactcca cagtctgata aataactaat taatcggacg
451   agaataaaga tagagtccca ttctacatgt taatattgac aacaaggaaa
501   tttatagtaa gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details