Match details

Record ID: DQ787742.1
Database: nt Go to original record
Description: Viola chaerophylloides voucher KWNU56516 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon: Viola chaerophylloides
Original size: 964 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2 560 + 559 bp

Amplicon: 559 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggtaatcc tgagccaaat ccgatttttt gaaatgaaaa aagtttatat
151   agttatatag acagaataaa taaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aataaatgga gttgactgtc ttgcattagt aaagtaaggg
251   aacctttcgt taaaattgcg gattcaaagt aaaggataac cctataaata
301   catatacgta ctgaaagact atctcaaata attaatgtaa ttatgaaaaa
351   taaaataaaa atattgaatc gatttcaagt tgaagaaaaa atcgattgat
401   caaatcattc actccacagt ctgataaata actaattaat cggacgagaa
451   taaagataga gtcccattct acatgttaat attgacaaca aggaaattta
501   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
551   tctatcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.52e-08
primerB 100.0 20 bp 0 0 559 540 2.52e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.