JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ787739.1
Database:	nt	Go to original record
Description:	Viola chaerophylloides voucher KWNU56520 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viola chaerophylloides
Original size:	950 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	553	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggcaatcc tgagccaaat ccgatttttt gaaatgaaaa aaagtttata
151   tagacagaat aaataaaaaa ggataggtgc agagactcaa tggaagctat
201   tctaataaat ggagttgact gtcttgcatt agtaaagtaa gggaaccttt
251   cgttaaaatt gcggattcaa agtaaaggat aaccctataa atacatatac
301   gtactgaaag actatctcaa ataattaatg taattatgaa aaataaaata
351   aaaatattga atcgatttca agttgaagaa aaaatcaatt gatcaaatca
401   ttcactccac agtctgataa ataactaatt aatcggacga gaataaagat
451   agagtcccat tctacatgtt aatattgaca acaaggaaat ttatagtaag
501   aggaaaatcc gtcgacttta gaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details