JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ787738.1
Database:	nt	Go to original record
Description:	Viola chaerophylloides voucher KWNU56517 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Viola chaerophylloides
Original size:	960 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	556	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tggataactt tcaaattcag agaaaccctg gaataaaaaa
101   ggggcaatcc tgagccaaat ccgctttttt gaaatgaaaa aaagtttata
151   tagacagaat aaataaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaataaat ggagttgact gtcttgcatt agtaaagtaa gggaaccttt
251   cgttaaaatt gcggattcaa agtaaaggat aaccctataa aaaatacata
301   tacgtactga aagactatct caaataatta atgtaattat gaaaaataaa
351   ataaaaatat tgaatcgatt tcaagttgaa gaaaaaatcg attgatcaaa
401   tcattcactc cacagtctga taaataacta attaatcgga cgagaataaa
451   gatagagtcc cattctacat gttaatattg acaacaagga aatttatagt
501   aagaggaaaa tccgtcgact ttagaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details