JRC GMO-Amplicons | EURL GMFF

Record ID:	EF177383.1
Database:	nt	Go to original record
Description:	Caesalpinia caladenia tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Erythrostemon caladenia
Original size:	1079 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	603	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaata agagttcaga
151   aagggagaat aaaaaaagga taggtgcaga gactcaacgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg ttagtaaagg aatccttcca
251   tcgaaactcc agaaaagaaa gaaaggatca aggatgaaca tatatatatg
301   tatactgaaa tattatttca attgattaga ccagacagac cccaaatccc
351   tattttttaa tatttatatg acaaatgaaa gatgtgaata gattccaagt
401   tgaagaaaga atcgaatatt tattgatcaa accattcatt ccatgataat
451   gatagtctga taaatctttt gaagaactga ttaatcggac gagaataaag
501   atagagtccc attctacatg tcaatacgac aacaattaaa tttatagtaa
551   gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details