JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266969.1
Database:	nt	Go to original record
Description:	Pentas parvifolia chloroplast trnT-L-F region, specimen voucher Luke 8340 (UPS)
Taxon:	Rhodopentas parvifolia
Original size:	1707 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	734	1324	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcggaa
151   agtgaaaaaa ggaaagggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttggctgcgg tagtcgagaa gtcttttcat caaaaattca
251   taaagtctaa gtagtaaaga tgaagtgaag gataaacgta tatacatacg
301   tagtaaatag tatattaaat gagtaatgac agcctgaatg aatctatatt
351   tttttctatg aacaaataaa gaattgttgt tagtagattc cgcgtttaag
401   aaagaatcaa atattcattg atcaaatgat tcactccata gtctgatcga
451   tcttttcacg aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa tgctggcaac aatgaaattt atagttaaga ggaaaatccg
551   tcgactttta aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details