JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266961.1
Database:	nt	Go to original record
Description:	Pentas ionolaena chloroplast trnL-F region, specimen voucher Borhidi, Hall, Hedberg & Mshoo 82250 (UPS)
Taxon:	Pentas ionolaena
Original size:	995 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcggaa
151   agtgaaaaaa tggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcggtagtc gagaagtctt ttcatcaaaa attcataaag
251   tctaagtatt aaagatgaag tgaaggataa acgtatatac atacgtagta
301   aatagtatat taaatgagta atgacagccc gaatgaatct atattttttc
351   tatgaacaaa tcaaataaag aattgttgtt agtagattcc gcgtttaaga
401   aagaatcgaa tattcattga tcaaatgatt cactccacag tctgatcgat
451   cttttcacga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat gccggcaaca atgaaattta tagttaaaag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details