JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266955.1
Database:	nt	Go to original record
Description:	Pentas caffensis chloroplast trnT-L-F region, specimen voucher Puff & Kelbessa 820811-2/2 (UPS)
Taxon:	Pentas caffensis
Original size:	1759 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	765	1354	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcggaa
151   agtgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcggtagtc gagaagtctt ttcatcaaaa atttataaag
251   tctaagtatt aaagatgaag tgaagtgaag gataaacgta tatacatacg
301   tagtaaatag tatattaaat gagtaatgac agcccgaatg aatctatatt
351   ttttctatga acaaataaag aattgttgtt agtggattcc gcgtttaaga
401   aagaatcgaa tgttcattga tcaaatgatt cactccatag tctgatcgat
451   cttttcacga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat gccggcaaca atgaaattta tagttaagag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details