JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266954.1
Database:	nt	Go to original record
Description:	Pentas bussei chloroplast trnT-L-F region, specimen voucher Luke 8319 (UPS)
Taxon:	Rhodopentas bussei
Original size:	1739 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	740	1329	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcggaa
151   agtgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcggtagtc gagaagcctt ttcattcatc aaaaattcat
251   aaagtctaag tagtaaagat gaagtgaagg ataaacgtat atctatacgt
301   agtaaatagt atattaaatg agtaatgaca gcccgaatga atctatattt
351   ttttctatga acaaataaag aattgttgtt agtagattcc gcgtttaaga
401   aagaatcaaa tattcattga tcaaatgatt cactccatag tctgatcgat
451   cttttcacca actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat gccggcaaca atgaaattta tagttaagag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details