JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266934.1
Database:	nt	Go to original record
Description:	Paraknoxia parviflora chloroplast trnT-L-F region, specimen voucher Verdcourt 2454 (S)
Taxon:	Paraknoxia parviflora
Original size:	1761 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	767	1361	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ctcctatttt ccgaaaacaa
151   aggttcggaa agtgaaaaaa gggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttggc tgcggtagtc gagacgtctt ttcatcaaaa
251   attcataaag tctaagtatt caagataaag tgaaggataa acgtatatac
301   atacgtagca aatagtatat taaatgagta atgacagttc caatgaatct
351   atattttttc tatgaacaaa taaagaattg ttgttagtcg attccgcgtt
401   taagaagaaa tcgactattc attgatcaaa agattcactc catagtctga
451   tcgatctttt cacgaactga ttaatcggat gagaataaag atagagtccc
501   attctacatg tcaatgccgg caacaatgaa atttatagtt aagaggaaaa
551   tccgtcgact tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details