JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266924.1
Database:	nt	Go to original record
Description:	Otiophora parviflora chloroplast trnT-L-F region, specimen voucher Cholocholo, Nurmi & Steiner 16 (UPS)
Taxon:	Otiophora parviflora
Original size:	1758 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	749	1358	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcttatttt ctcctatttt ccgaaaacaa
151   aacaaaggtt cgaaaagtga aaatggaaaa agggataggt gcagagactc
201   aatggaagct gttctaacaa atggagttgg ctgcggtaat cgagaagttt
251   tttcatcaaa aattcataaa gtctaagtat tcaagatgga gtgaagtgaa
301   ggataaacgt atatacatac gtagtaaata gtatattaaa tgagtaatga
351   cagcccgaat gaatctatat tttttctatg aacaaataaa gaattgttgt
401   tagtagattc cgcgtttaag aaagaatcga atattcattg atcaaaagat
451   tcactccata gtctgatcga tcttttcacg aatgattaat cggacgagaa
501   taaagataga gtcccattct acatgtcaat gccggcaaca atgaaattta
551   tagttaaaag gaaaatccgt cgacttttaa aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details