JRC GMO-Amplicons | EURL GMFF

Record ID:	AM266913.1
Database:	nt	Go to original record
Description:	Chlorochorion monticola chloroplast trnT-L-F region, specimen voucher Stolz 1286 (S)
Taxon:	Chlorochorion monticola
Original size:	1737 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	743	1337	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ctcctatttt ccgaaaacaa
151   aggttcggaa agtgaaaaaa gggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttggc tgcggtagtc gagacgtctt ttcatcaaaa
251   attcataaag tctaagtatt caagatgaag tgaaggataa acgtatatac
301   atacgtagta actagtatat taaatgagta atgacagccc gaatgaatct
351   atattttttc tatgaacaaa taaagaatta ttgttattcg attccgcgtt
401   taagaaagaa tcgaatattc attgatcaaa agattcactc catagtctga
451   tcgatctttt cacgaactga ttaatcggat gagaataaag atagagtccc
501   attctacatg tcaatgccgg caacaatgaa atttatagtt aagaggaaaa
551   tccgtcgact tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details