JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ839446.1
Database:	nt	Go to original record
Description:	Phtheirospermum japonicum isolate 05360 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phtheirospermum japonicum
Original size:	1665 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	772	1326	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgatt gcgtactaga ggaatgaaac ttcagaaagg atgaaggata
251   aacgtatcta ttgaatacta tatcaaatga ttgattaatg gtggcccgaa
301   tctgtatctg tatttttgaa taccaaaaat ggaagaattg gtgtgaattg
351   attccatatt gaaattgaac aaagaatatt cattcattaa atcattcact
401   ccatagtccg atagatcttt taaagaactg attaatcgga cgagaataaa
451   gatagagtcc cattctacat gtcaataccg gcaacaatga aatttatagt
501   aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details