JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ418003.1
Database:	nt	Go to original record
Description:	Gustavia dubia voucher Marianne Akers 7 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Gustavia dubia
Original size:	1009 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	582	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   ataaagcgaa aataaaaaaa aaaaggatag gtgcagagac tcaatggaag
201   ctgttctaac aaatggagtt gactgcgttg gtagaggaat ctttccatcg
251   aaacttcaga aaggatgaaa gagaaaccta tatacatagg caaactactg
301   aaatgctata tcaaatgatt aatgacggcc aaaatctgat ccgtattttt
351   ttatatttaa aatgtaagaa ttgttgtaaa tcgattccac ataaagaatt
401   gaatatttat tgatcaaatc atttactcca tggtctaata gatcttttga
451   agagctgatt aatcggacga gaataaagat agagtcccat tctacatgtc
501   aataccgaca acaatgaaat ttatagtaag aggaaaatcc gttgactttc
551   aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details