JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ417969.1
Database:	nt	Go to original record
Description:	Eschweilera mexicana voucher Wendt et al. 4180 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Eschweilera mexicana
Original size:	1025 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	598	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   ataaagcgaa aataaaaaaa aaaaaggata ggtgcagaga ctcaatggaa
201   gctgttctaa caaatggagt tgactgcgtt ggtagaggaa tctttccatc
251   gaaacttcag aaaggatgaa agagaaacct atatacatag acagactact
301   gaaatactat atcaaatgat taatgacggc caaaatctgg atccgtattt
351   tgtatttttt tatatttaaa atttaaaatg gaagaattgt tgggaatcga
401   ttccacataa agaattgaat atttattgat caaatcattt actccatggt
451   ctgatagatc ttttgaagag ctgattaatc ggacgagaat aaagatagag
501   tcccattcta catgtcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgttg actttcaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details