JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ417957.1
Database:	nt	Go to original record
Description:	Eschweilera atropetiolata voucher Pacheco et al. 91 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Eschweilera atropetiolata
Original size:	1020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	594	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   ataaagcgaa aataaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaatctt tccatcgaaa
251   cttcagaaag gatgaaagag aaacctatat acatagacag actactgaaa
301   tactatatca aatgattaat gacggccaaa atctggatcc atattttgta
351   tttttttata tttaaaattt aaaatggaag aattgttggg aatcgattcc
401   acataaagaa ttgaatattt attgatcaaa tcatttactc catggtctga
451   tagatctttt gaagagctga ttaatcggac gagaataaag atagagtccc
501   attctacatg tcaataccga caacaatgaa atttatagta agaggaaaat
551   ccgttgactt tcaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details