JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ417955.1
Database:	nt	Go to original record
Description:	Eschweilera amazoniciformis voucher Nascimento et al. 644 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Eschweilera amazoniciformis
Original size:	991 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   ataaagcgaa aataaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaatctt tccatcgaaa
251   cttcagaaag gatgaaagag aaacctatat acataggcag actactgaaa
301   tactatatca aatgattaat gacggccaaa atctggatcc gtattttttt
351   atatttaaaa tggaagaatt aaatggaaga attgttggga atcgattcca
401   cataaagaat tgaatattta ttgatcaaat catttactcc atggtctgat
451   agatcttttg aagagctgat taatcggacg agaataaaga tagagtccca
501   ttctacatgt caataccaac aacaatgaaa tttatagtaa gaggaaaatc
551   cgttgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details