JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ417936.1
Database:	nt	Go to original record
Description:	Cariniana domestica voucher Solomon 7848 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cariniana domestica
Original size:	997 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	567	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gagcttaatt ggattgagcc ttggtatgga
51    aacctactaa gtgataactt tcaaattcag agaaaccctg gaattaataa
101   aaatgggcaa tcctgagcca aatcctgttt ttcgaaaaca aacaaagatt
151   cataaagcga aaataaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggga atctttccat cgaaacttca
251   gaaagaatga aagagaaacc tatatacata ggcagactac tgaaatacta
301   tatcaaatga ttaatgacgg ccaaaatctg gatccgtatt ttaaatggaa
351   gaattgttgt gaatcgattc cacataaaga attgaatatt tattgatcaa
401   atcatttact ccatggtctg atagatcttt tgaagagctg attaatcgga
451   cgagtaataa agatagagtc ccattctaca tgtcaatacc cgacaacaat
501   gaaatttata gtaagaggaa aatccgttga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details