Match details

Record ID: NRN_FW366933
Database: nrnl1 Go to original record
Description: MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 186 from Patent WO2008073271. Sequence 186 from Patent WO2008073275. Sequence 186 from patent US 7709239. Sequence 186 from patent US 8017838. Sequence 186 from patent US 8026089.
Taxon: unidentified
Original size: 11766 bp
Patents: JP2010512146 (lens, espacenet) WO2008073271 (lens, espacenet) WO2008073275 (lens, espacenet) US7709239 (lens, espacenet) US8017838 (lens, espacenet) US8026089 (lens, espacenet)

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Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3856 3973 - 118 bp

Amplicon: 118 bp
1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc 

View other 15178 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.22e-11
primerB 100.0 24 bp 0 0 118 95 4.22e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.