JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_FW366933
Database:	nrnl1	Go to original record
Description:	MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 186 from Patent WO2008073271. Sequence 186 from Patent WO2008073275. Sequence 186 from patent US 7709239. Sequence 186 from patent US 8017838. Sequence 186 from patent US 8026089.
Taxon:	unidentified
Original size:	11766 bp
Patents:	JP2010512146 (lens, espacenet) WO2008073271 (lens, espacenet) WO2008073275 (lens, espacenet) US7709239 (lens, espacenet) US8017838 (lens, espacenet) US8026089 (lens, espacenet)

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Method:	QL-ELE-00-001	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	5269	5463	-	195 bp

Amplicon:

195 bp

1     gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgacatctc cactgacgta agggatgacg cacaatccca ctatc

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	19 bp	0	0	1	19	3.10e-08
primerB	100.0	20 bp	0	0	195	176	9.28e-09

Match details