Match details

Record ID: NRN_FB706328
Database: nrnl1 Go to original record
Description: Sequence 35 from Patent WO2007127381. Sequence 81 from Patent WO2007136876. Sequence 81 from Patent WO2007136877. MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Delta-9 Elongases and Their Use in Making Polyunsaturated Fatty Acids. Sequence 79 from Patent WO2008063340. Sequence 189 from Patent WO2008073271. Sequence 189 from Patent WO2008073275. Sequence 45 from Patent WO2008100545. Sequence 115 from Patent WO2008124048. Sequence 6 from Patent WO2008128240. Sequence 15 from Patent WO2008128241. Sequence 189 from patent US 7709239. Sequence 15 from patent US 7794701. Sequence 79 from patent US 7863502. Sequence 35 from patent US 7943823. Sequence 189 from patent US 8017838. Sequence 189 from patent US 8026089. Sequence 45 from patent US 8013215. Sequence 81 from patent US 8049070. Sequence 35 from patent US 8058517. Sequence 6 from patent US 8119860. Sequence 42 from patent US 8188338. Sequence 15 from patent US 8298797. Sequence 42 from Patent WO2008124806. Sequence 29 from Patent WO2008137516. Sequence 35 from Patent EP2405012. Sequence 29 from Patent EP2471928.
Taxon: unidentified
Original size: 4311 bp
Patents: WO2007127381 (lens, espacenet) WO2007127381 (lens, espacenet) WO2007136876 (lens, espacenet) WO2007136876 (lens, espacenet) WO2007136877 (lens, espacenet) WO2007136877 (lens, espacenet) JP2010512146 (lens, espacenet) JP2010523113 (lens, espacenet) JP2010524468 (lens, espacenet) WO2008063340 (lens, espacenet) WO2008073271 (lens, espacenet) WO2008073275 (lens, espacenet) WO2008100545 (lens, espacenet) WO2008124048 (lens, espacenet) WO2008128240 (lens, espacenet) WO2008128241 (lens, espacenet) US7709239 (lens, espacenet) US7794701 (lens, espacenet) US7863502 (lens, espacenet) US7943823 (lens, espacenet) US8017838 (lens, espacenet) US8026089 (lens, espacenet) US8013215 (lens, espacenet) US8049070 (lens, espacenet) US8058517 (lens, espacenet) US8119860 (lens, espacenet) US8188338 (lens, espacenet) US8298797 (lens, espacenet) WO2008124806 (lens, espacenet) WO2008137516 (lens, espacenet) EP2405012 (lens, espacenet) EP2471928 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2887 3101 - 215 bp

Amplicon: 215 bp
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.