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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FB572986
Database: nrnl1 Go to original record
Description: Sequence 3 from Patent WO2006130436. SOYBEAN EVENT MON89788 AND METHODS FOR DETECTION THEREOF. Sequence 3 from patent US 7632985. Sequence 3 from patent US 8053184. Sequence 3 from Patent EP2275561.
Taxon: unidentified
Original size: 1222 bp
Patents: WO2006130436 (lens, espacenet) WO2006130436 (lens, espacenet) JP2008545413 (lens, espacenet) US7632985 (lens, espacenet) US8053184 (lens, espacenet) EP2275561 (lens, espacenet)
Method: QT-EVE-GM-006 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event MON89788 (Charles Delobel et al., 2008).
Target:MON 89788

View other 23 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1059 1197 + 139 bp
Amplicon: 139 bp 
1     tcccgctcta gcgcttcaat cgtggttatc aagctccaaa cactgatagt
51    ttaaactgaa ggcgggaaac gacaatctga tccccatcaa gctctagcta
101   gagcggccgc gttatcaagc ttctgcaggt cctgctcga

View other 23 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 7.05e-09
primerB 100.0 19 bp 0 0 139 121 2.37e-08
probe 100.0 24 bp 0 0 56 79 5.02e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.