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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_AX828526
Database: nrnl1 Go to original record
Description: Sequence 172 from Patent WO03074073. Sequence 172 from Patent EP1566387. Genes implicated in the regulation of angiogenesis, pharmaceutical preparations containing them and their applications. GENES INVOLVED IN REGULATING ANGIOGENESIS, PHARMACEUTICAL PREPARATIONS CONTAINING SAME AND APPLICATIONS THEREOF. Sequence 172 from patent US 7553492. Sequence 172 from patent US 8133877. Sequence 172 from Patent EP2161280.
Taxon: unidentified
Original size: 5841 bp
Patents: WO03074073 (lens, espacenet) EP1566387 (lens, espacenet) JP2009050262 (lens, espacenet) US7553492 (lens, espacenet) US2005153917 (lens, espacenet) US8133877 (lens, espacenet) EP2161280 (lens, espacenet)

View other 1 match for this record

Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

View other 58813 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3131 3303 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.