JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_AX765966
Database:	nrnl1	Go to original record
Description:	Sequence 52 from Patent WO02097097. Sequence 58 from Patent WO2004020642. Sequence 52 from patent US 7172881. Sequence 58 from patent US 7262338. Sequence 52 from patent US 8030470. Sequence 52 from Patent EP2123763.
Taxon:	unidentified
Original size:	5715 bp
Patents:	WO02097097 (lens, espacenet) WO2004020642 (lens, espacenet) US7172881 (lens, espacenet) US2003167535 (lens, espacenet) US7262338 (lens, espacenet) US2004010821 (lens, espacenet) US8030470 (lens, espacenet) EP2123763 (lens, espacenet)

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Method:	QL-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	3189	3311	+	123 bp

Amplicon:

123 bp

1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gaa

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	1.84e-09
primerB	100.0	23 bp	0	0	121	99	1.68e-10

Match details