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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FW366939
Database: nrnl1 Go to original record
Description: MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 192 from Patent WO2008073271. Sequence 192 from Patent WO2008073275. Sequence 192 from patent US 7709239. Sequence 192 from patent US 8017838. Sequence 192 from patent US 8026089.
Taxon: unidentified
Original size: 11752 bp
Patents: JP2010512146 (lens, espacenet) WO2008073271 (lens, espacenet) WO2008073275 (lens, espacenet) US7709239 (lens, espacenet) US8017838 (lens, espacenet) US8026089 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 5245 5323 - 79 bp
Amplicon: 79 bp 
1     cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga

View other 3624 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.41e-10
primerB 100.0 22 bp 0 0 79 58 3.41e-10
probe 100.0 26 bp 0 0 31 56 2.52e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.