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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_A18053
Database: nrnl1 Go to original record
Description: DNA for transforming monocotyledonous plants Sequence 1 from Patent WO9321335. Sequence 4 from Patent EP0955371. Sequence 4 from patent US 6002070. Sequence 4 from patent US 6074877. Sequence 4 from patent US 5641664. Sequence 1 from patent US 5679558. Sequence 4 from patent US 5712135.
Taxon: unidentified
Original size: 4883 bp
Patents: WO9209696 (lens, espacenet) WO9321335 (lens, espacenet) EP0955371 (lens, espacenet) US6002070 (lens, espacenet) US6074877 (lens, espacenet) US5641664 (lens, espacenet) US5679558 (lens, espacenet) US5712135 (lens, espacenet)

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Method: QL-ELE-00-026 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1870 1929 + 60 bp
Amplicon: 60 bp 
1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
51    ggacgacctc

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Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.06e-08
primerB 100.0 18 bp 0 0 60 43 3.56e-08
probe 100.0 18 bp 0 0 21 38 3.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.