European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_EA247437
Database: nrnl1 Go to original record
Description: Sequence 10 from patent US 7227011. NUCLEIC ACID VACCINES FOR PREVENTION OF FLAVIVIRUS INFECTION. NUCLEIC ACID VACCINES FOR PREVENTION OF FLAVIVIRUS INFECTION. Sequence 10 from Patent EP1935991. Sequence 10 from patent US 7521177. Sequence 10 from patent US 7632510. Sequence 10 from patent US 7662394. Sequence 10 from patent US 8221768. Sequence 10 from patent US 8232379. JP 2010017185-A/7: Nucleic Acid Vaccines for Prevention of Flavivirus Infection.
Taxon: unidentified
Original size: 7500 bp
Patents: US7227011 (lens, espacenet) US2003022849 (lens, espacenet) JP2004532023 (lens, espacenet) EP1935991 (lens, espacenet) US7521177 (lens, espacenet) US2007166701 (lens, espacenet) US7632510 (lens, espacenet) US7662394 (lens, espacenet) US8221768 (lens, espacenet) US8232379 (lens, espacenet) JP2010017185 (lens, espacenet)

View other 1 match for this record

Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

View other 59426 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4519 4733 + 215 bp
Amplicon: 215 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
201   gccaggctca aggcg

View other 57490 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 3.06e-09
primerB 100.0 21 bp 0 0 215 195 3.06e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.