Match details
Record ID:
NRN_A60109
Database:
nrnl1
Go to original record
Description:
Sequence 2 from Patent WO9706267. Sequence 2 from Patent EP0757102. Sequence 2 from Patent EP1496125. Sequence 2 from patent US 6074876. GENETIC TRANSFORMATION USING A PARP INHIBITOR. Mutated barnase gene and transgenic plant thereof. MUTATED BARNASE GENE AND PLANT TRANSFORMED BY THE SAME.
Taxon:
unidentified
Original size:
6548 bp
Patents:
WO9706267 (lens , espacenet )
EP0757102 (lens , espacenet )
EP1496125 (lens , espacenet )
US6074876 (lens , espacenet )
JP2007089587 (lens , espacenet )
JP2000041682 (lens , espacenet )
View other 15 matches for this record
Method:
QL-ELE-00-013
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008).
Target: T-nos
View other 15584 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
2079
2162
-
84 bp
1 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51 cgcaattata catttaatac gcgatagaaa acaa
View other 30799 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
25 bp
0
0
1
25
9.20e-12
primerB
100.0
25 bp
0
0
84
60
9.20e-12
probe
100.0
28 bp
0
0
28
55
2.28e-13
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.