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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_DL477020
Database: nrnl1 Go to original record
Description: MODIFICATION OF PLANT LIGNIN CONTENT. Sequence 14 from patent US 7402428. Sequence 14 from patent US 7456338. Sequence 14 from patent US 7807880. Sequence 14 from patent US 8030545. JP 2011055839-A/14: MODIFICATION OF PLANT LIGNIN CONTENT.
Taxon: unidentified
Original size: 3799 bp
Patents: JP2008513025 (lens, espacenet) US7402428 (lens, espacenet) US2006101535 (lens, espacenet) US7456338 (lens, espacenet) US2006130183 (lens, espacenet) US7807880 (lens, espacenet) US8030545 (lens, espacenet) JP2011055839 (lens, espacenet)

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Method: QL-ELE-00-009 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Lipp et al.,2001).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3663 3780 + 118 bp
Amplicon: 118 bp 
1     gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
51    tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
101   attatcgcgc gcggtgtc

View other 15178 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 24 bp 0 0 1 24 4.22e-11
primerB 100.0 24 bp 0 0 118 95 4.22e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.