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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_EA059901
Database: nrnl1 Go to original record
Description: Sequence 5 from patent US 7166771. COORDINATED DECREASE AND INCREASE OF GENE EXPRESSION OF MORE THAN ONE GENE USING TRANSGENIC CONSTRUCTS. COORDINATED DECREASE AND INCREASE OF GENE EXPRESSION OF MORE THANONE GENE USING TRANSGENIC CONSTRUCTS. Sequence 5 from patent US 7795504.
Taxon: unidentified
Original size: 8179 bp
Patents: US7166771 (lens, espacenet) US2004126845 (lens, espacenet) JP2007506437 (lens, espacenet) US7795504 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6549 6627 + 79 bp
Amplicon: 79 bp 
1     cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga

View other 6569 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.41e-10
primerB 100.0 22 bp 0 0 79 58 3.41e-10
probe 100.0 26 bp 0 0 31 56 2.52e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.