JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_DD123296
Database:	nrnl1	Go to original record
Description:	Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes. CHROMOSOME-BASED PLATFORMS. CHROMOSOME-BASED PLATFORMS. Sequence 92 from patent US 7521240. JP 2009254382-A/3: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes.
Taxon:	unidentified
Original size:	10549 bp
Patents:	JP2005500827 (lens, espacenet) JP2005503778 (lens, espacenet) JP2009017884 (lens, espacenet) US7521240 (lens, espacenet) US2005181506 (lens, espacenet) JP2009254382 (lens, espacenet)

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Method:	QL-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	10410	10532	+	123 bp

Amplicon:

123 bp

1     ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gaa

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	1.84e-09
primerB	100.0	23 bp	0	0	121	99	1.68e-10

Match details