JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_DD123294
Database:	nrnl1	Go to original record
Description:	Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes. CHROMOSOME-BASED PLATFORMS. CHROMOSOME-BASED PLATFORMS. Sequence 89 from patent US 7521240. JP 2009254382-A/1: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes.
Taxon:	unidentified
Original size:	11182 bp
Patents:	JP2005500827 (lens, espacenet) JP2005503778 (lens, espacenet) JP2009017884 (lens, espacenet) US7521240 (lens, espacenet) US2005181506 (lens, espacenet) JP2009254382 (lens, espacenet)

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Method:	QL-ELE-00-005	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	0	7110	7304	-	195 bp

Amplicon:

195 bp

1     gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
51    gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
151   gtgatatctc cactgacgta agggatgacg cacaatccca ctatc

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	94.7	19 bp	1	0	1	19	1.44e-06
primerB	100.0	20 bp	0	0	195	176	9.28e-09

Match details