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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_DD123308
Database: nrnl1 Go to original record
Description: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes. Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes. CHROMOSOME-BASED PLATFORMS. CHROMOSOME-BASED PLATFORMS. CHROMOSOME-BASED PLATFORMS. CHROMOSOME-BASED PLATFORMS. JP 2010529845-A/30: Protein Production in Plants. Sequence 104 from patent US 7521240. Sequence 107 from patent US 7521240. Sequence 2824 from Patent WO2009083958. Sequence 922 from Patent EP2154946. Sequence 34 from Patent EP2171068. Sequence 1651 from Patent EP2183371. JP 2009254382-A/15: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes. JP 2009254382-A/17: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes.
Taxon: unidentified
Original size: 277 bp
Patents: JP2005500827 (lens, espacenet) JP2005500827 (lens, espacenet) JP2005503778 (lens, espacenet) JP2005503778 (lens, espacenet) JP2009017884 (lens, espacenet) JP2009017884 (lens, espacenet) JP2010529845 (lens, espacenet) US7521240 (lens, espacenet) US2005181506 (lens, espacenet) US7521240 (lens, espacenet) US2005181506 (lens, espacenet) WO2009083958 (lens, espacenet) EP2154946 (lens, espacenet) EP2171068 (lens, espacenet) EP2183371 (lens, espacenet) JP2009254382 (lens, espacenet) JP2009254382 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 166 234 + 69 bp
Amplicon: 69 bp 
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.