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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_DD123313
Database: nrnl1 Go to original record
Description: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes. CHROMOSOME-BASED PLATFORMS. CHROMOSOME-BASED PLATFORMS. Sequence 109 from patent US 7521240. JP 2009254382-A/20: Plant Artificial Chromosomes, Uses thereof, and Methods of Preparing Plant Artificial Chromosomes.
Taxon: unidentified
Original size: 14627 bp
Patents: JP2005500827 (lens, espacenet) JP2005503778 (lens, espacenet) JP2009017884 (lens, espacenet) US7521240 (lens, espacenet) US2005181506 (lens, espacenet) JP2009254382 (lens, espacenet)

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Method: QL-ELE-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase gene (Grohmann et al., 2009).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 6858 6917 - 60 bp
Amplicon: 60 bp 
1     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac
51    ggacgacctc

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Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.06e-08
primerB 100.0 18 bp 0 0 60 43 3.56e-08
probe 100.0 18 bp 0 0 21 38 3.56e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.