Match details
Record ID: NRN_EA059902
Database:
nrnl1
Go to original record
Description:
Sequence 6 from patent US 7166771. COORDINATED DECREASE AND INCREASE OF GENE EXPRESSION OF MORE THAN ONE GENE USING TRANSGENIC CONSTRUCTS. COORDINATED DECREASE AND INCREASE OF GENE EXPRESSION OF MORE THANONE GENE USING TRANSGENIC CONSTRUCTS. Sequence 6 from patent US 7795504.
Taxon:
unidentified
Original size:
7713 bp
Patents:
US7166771 (lens , espacenet )
US2004126845 (lens , espacenet )
JP2007506437 (lens , espacenet )
US7795504 (lens , espacenet )
View other 15 matches for this record
Method: QL-ELE-00-012
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target: CaMV P-35S
View other 15625 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
6007
6088
+
82 bp
1 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 26489 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
18 bp
0
0
1
18
4.54e-08
primerB
100.0
22 bp
0
0
82
61
3.55e-10
probe
100.0
22 bp
0
0
21
42
3.55e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
